Synergy labs grey tn2/2/2024 ![]() SARS-CoV-2 and SARS-CoV, the genomes of which share approximately 78% sequence identity 1, both use human ACE2 as an entry receptor 7, 8, 9. The S1 subunit contains an N-terminal domain (NTD) and a receptor-binding domain (RBD). The S protein is composed of an N-terminal subunit (S1) that mediates receptor binding, and a C-terminal subunit (S2) that mediates fusion between the virus and the membrane of the host cell. The S protein of SARS-CoV-2 is the molecular determinant of viral attachment, fusion and entry into host cells 6. These results identify protective epitopes on the S RBD and provide a structure-based framework for rational vaccine design and the selection of robust immunotherapeutic agents. In addition, passive transfer of either of two of the most potent ACE2-blocking monoclonal antibodies (COV2-2196 or COV2-2381) as monotherapy protected rhesus macaques from SARS-CoV-2 infection. In two mouse models of SARS-CoV-2 infection, passive transfer of COV2-2196, COV2-2130 or a combination of both of these antibodies protected mice from weight loss and reduced the viral burden and levels of inflammation in the lungs. Two potently neutralizing monoclonal antibodies, COV2-2196 and COV2-2130, which recognize non-overlapping sites, bound simultaneously to the S protein and neutralized wild-type SARS-CoV-2 virus in a synergistic manner. Using competition-binding, structural and functional studies, we show that the monoclonal antibodies can be clustered into classes that recognize distinct epitopes on the S RBD, as well as distinct conformational states of the S trimer. Here we analyse a large panel of human monoclonal antibodies that target the spike (S) glycoprotein 5, and identify several that exhibit potent neutralizing activity and fully block the receptor-binding domain of the S protein (S RBD) from interacting with human angiotensin-converting enzyme 2 (ACE2). Moreover, we currently lack a thorough understanding of the mechanisms of humoral immunity to SARS-CoV-2 4. The ongoing pandemic of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a major threat to global health 1 and the medical countermeasures available so far are limited 2, 3. Nature volume 584, pages 443–449 ( 2020) Cite this article License No.: 0000002128 Virginia Dickenson County Community HospitalĬLIA No.: 49D0232228 Johnston Memorial HospitalĬLIA No.: 49D0232174 Norton Community HospitalĬLIA No.: 49D0666681 Russell County Medical CenterĬLIA No.: 49D0232283 Smyth County Community HospitalĬopies of all certificates are available by contacting (423) 302-3707.Potently neutralizing and protective human antibodies against SARS-CoV-2 License No.: 0000003343 Unicoi County Memorial HospitalĬLIA No.: 44D0028470 Sycamore Shoals Hospital License No.: 0000002176 Johnson County Health Care Center License No.: 0000002212 Johnson City Medical Center License No.: 0000004192 Indian Path Medical Center Accreditations and certifications Tennessee Franklin Woods Community HospitalĬlinical Laboratory Improvement Act (CLIA) ![]() Synergy Laboratories is proud to offer, through Ballad Health laboratories, comprehensive clinical laboratory testing to physicians to aid in diagnosing and/or treating disease or injury.
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